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noti digested pcmv vector  (TaKaRa)


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    TaKaRa noti digested pcmv vector
    Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the <t>pCMV</t> control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.
    Noti Digested Pcmv Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 133 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/noti digested pcmv vector/product/TaKaRa
    Average 93 stars, based on 133 article reviews
    noti digested pcmv vector - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "Distinct regulatory elements of SLC6A14 expression contribute to modification of cystic fibrosis phenotypes"

    Article Title: Distinct regulatory elements of SLC6A14 expression contribute to modification of cystic fibrosis phenotypes

    Journal: Human Genetics

    doi: 10.1007/s00439-025-02800-7

    Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the pCMV control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.
    Figure Legend Snippet: Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the pCMV control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.

    Techniques Used: Activity Assay, Luciferase, Plasmid Preparation, Cotransfection, Control



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    TaKaRa noti digested pcmv vector
    Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the <t>pCMV</t> control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.
    Noti Digested Pcmv Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/noti digested pcmv vector/product/TaKaRa
    Average 93 stars, based on 1 article reviews
    noti digested pcmv vector - by Bioz Stars, 2026-06
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    ecori noti digested pcmv myc mammalian expression vector  (TaKaRa)
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    Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the <t>pCMV</t> control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.
    Ecori Noti Digested Pcmv Myc Mammalian Expression Vector, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the pCMV control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.

    Journal: Human Genetics

    Article Title: Distinct regulatory elements of SLC6A14 expression contribute to modification of cystic fibrosis phenotypes

    doi: 10.1007/s00439-025-02800-7

    Figure Lengend Snippet: Distal enhancer activity with augmented response to IRF1 depending on rs4446858 SNP allele. A Juxtaposition of the 2.1 kb segment of the 17 kb sub-region in forward (F) or reverse (R) orientation to the core promoter increased reporter gene activity in MOR cells, but not in CFPAC-1 cells. Luciferase activity values were determined as in Fig. , except that activity of core promoter only was set as 1 for comparisons. Two independent experiments were performed, each with two to four technical replicates for each vector. B The 2.1 kb segment with the most common SNP combination (haplotype D1; black), with alternate alleles A and C for rs12009976 and rs4446858, respectively (haplotype D4; pale) and with the alternate C allele for rs4446858 only (grey) yielded comparable reporter gene activities. Each bar represents the mean of five to ten replicates from two independent experiments. C Inclusion of exogenous IRF1 by co-transfection revealed further enhancement of the reporter gene activity with the alternate C allele of rs4446858. The exogenous inclusion of IRF2 modestly reduced enhancer activity, compared to the pCMV control vector only, and was independent of the C allele. A combination of IRF2 with IRF1 reduced the effect of IRF1 alone. The reporter gene assays were performed as in ( A ) except the core promoter fragment with pGL4.10 plus pCMV was set as 1 for comparisons. Each bar represents mean of six to twelve replicates from two independent experiments. For all experiments error bars represent SEM. * P < 0.05, ** P < 0.01, *** P < 0.001 using unpaired t tests.

    Article Snippet: The SLC6A14 insert was ligated to the EcoRI/BamHI-digested pTetOne vector (Clontech, 634310), and IRF1 , IRF2 inserts were ligated to the NotI-digested pCMV vector (Clontech, 631719), replacing LacZ .

    Techniques: Activity Assay, Luciferase, Plasmid Preparation, Cotransfection, Control